Figure 3.

Differential recruitment of MLR subpopulations during behavior

(A) Approach for in vivo calcium imaging of MLR>SC and MLR-Rbp4 neurons.

(B) Fraction of time mice spend sedentary, walking, rearing, handling or grooming in the open field (n = 11).

(C) Distribution for lengths of locomotor, rearing, grooming, handling episodes observed in open field (kernel density estimate [KDE] density; dashed lines: median of distribution).

(D) Z-scored fluorescence with overlaid rearing episodes from two rearing-tuned example neurons.

(E) Baseline (BL) subtracted average fluorescence (±SEM) of all MLR>SC neurons aligned to behavioral onset (56 neurons, n = 7 mice).

(F) Graphs depicting mean evoked fluorescence during rearing, walking, grooming, and handling of all MLR>SC neurons in rising order (colored dots, positively modulated neurons; gray dots, all others).

(G) KDE density of neurons from graphs shown in (F).

(H) Number of positively modulated MLR>SC neurons across behaviors (Venn diagrams). Comparison between forelimb and full-body behaviors (top) and between the full-body behaviors rear and walk (bottom).

(I) Z-scored fluorescence with overlaid grooming and handling episodes from two MLR-Rbp4 example neurons.

(J) Baseline (BL) subtracted average fluorescence (±SEM) of all MLR-Rbp4 neurons aligned to behavioral onset (152 neurons, n = 4 mice).

(K) Graphs depicting mean evoked fluorescence during rearing, walking, grooming, and handling of MLR-Rbp4 neurons in rising order (colored dots, positively modulated neurons; gray dots, all others).

(L) KDE density of neurons from the graphs shown in (K).

(M) Number of positively modulated MLR-Rbp4 neurons across different behaviors (Venn diagrams). Comparison between forelimb and full-body behaviors (top) and the forelimb behaviors groom and handle (bottom).

See also Figures S3 and S4.