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. 2021 Aug 19;184(17):4564–4578.e18. doi: 10.1016/j.cell.2021.07.002

Figure 6.

Figure 6

Opposite perturbation of MLR-Rbp4 neurons elicits pro- and antikinetic modulation of behavior

(A) Approach to target the optogenetic inhibitor stGtACR2 into MLR-Rbp4 neurons for LOF experiments (n = 10).

(B) DeepLabCut tracking of body part position (list shown to the right) upon optogenetic inhibition of MLR-Rbp4 neurons through laser application (white points before stimulation; orange to yellow points from 0 to 500 ms of laser stimulation), showing three single trials from one mouse.

(C) Average speed of body parts (±SEM) upon optogenetic inhibition (blue window) of MLR-Rbp4 neurons and reliability of laser induced speed increase (n = 10) compared to the probability of increase in speed in control mice upon light application (n = 5).

(D) Approach to target the optogenetic activator ReaChR into MLR-Rbp4 neurons for GOF experiments (n = 10).

(E) DeepLabCut tracking of body part position (list shown in B) upon optogenetic activation of MLR-Rbp4 neurons through laser application (white points before stimulation; orange to yellow points from 0 to 500 ms of laser stimulation), showing three single trials during rearing, grooming, or handling.

(F) Average (±SEM) of center-of-body-mass speed upon closed loop optogenetic activation (blue window) of MLR-Rbp4 neurons during locomotion and control no laser trials (black) with single-mouse averages of the two conditions (n = 10). Graph to the right shows reliability in speed decrease upon optogenetic activation of MLR-Rbp4 neurons compared to the probability of observing a locomotor speed decrease in control mice expressing GFP upon light application (n = 5).

(G–I) Average (±SEM) of speed of body parts (as indicated: forelimbs, hindlimbs, genitals, tail base, and snout) for rearing (left), grooming (middle), and handling (right) trials (n = 10). Graphs to the right show reliability in speed decrease upon optogenetic activation of MLR-Rbp4 neurons compared to the probability of observing a decrease in speed in control mice upon light application (n = 5).

See also Figure S7.

∗∗p ≤ 0.01.