Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Adv Nanobiomed Res. 2021 Mar 13;1(8):2100021. doi: 10.1002/anbr.202100021

Figure 5. Long-term immune memory effects of CpG-PBNP-PTT.

Figure 5.

(A) Representative scatter plots of central memory (TCM) and effector memory (TEM) lymphocytes in the CD4+ subset of T cells isolated from the spleens of PBNP-PTT and CpG-PBNP-PTT rechallenged mice compared to age-matched, treatment-naive control mice. (B) %CD4+ central memory (TCM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to mice in the control group. (C) %CD4+ effector memory (TEM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. (D) Representative scatter plots of central memory (TCM) and effector memory (TEM) lymphocytes in the CD8+ subset of T cells isolated from the spleen of PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. (E) %CD8+ central memory (TCM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. (F) %CD8+ effector memory (TEM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. (G) Representative scatter plots of central memory (TCM) and effector memory (TEM) lymphocytes in the CD4+ subset of T cells isolated from the spleen of PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control mice. (H) %CD4+ central memory (TCM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control mice. (I) %CD4+ effector memory (TEM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control mice. (J) Representative scatter plots and histograms of central memory (TCM) and effector memory (TEM) lymphocytes in the CD8+ subset of T cells isolated from the spleen of PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control mice. (K) %CD8+ central memory (TCM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control mice. (L) %CD8+ effector memory (TEM) in the PBNP-PTT and CpG-PBNP-PTT rechallenged mice upon co-culture with 9464D cells ex vivo as compared to control imce. (M) Representative scatter plots of GD2+ live 9464D cells from co-cultures with splenic T cells from PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. (N) %GD2+ cells from co-cultures with splenic T cells from PBNP-PTT and CpG-PBNP-PTT rechallenged mice as compared to control mice. Results are expressed as mean ± std. deviation; treatment-naive control (n=4), PBNP-PTT (n=2), CpG-PBNP-PTT (n=4) for spleen samples and CpG-PBNP-PTT (n=3) for co-culture samples; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (ANOVA).