Skip to main content
. 2021 Jul 21;32(8):1742–1752. doi: 10.1021/acs.bioconjchem.1c00260

Figure 1.

Figure 1

Schematic representation of the modified membrane scaffold protein sequence comprising a removable purification tag (His6 + TEV), two R5 peptides, and the MSP (a); process of generating silica-stabilized nanodiscs (b); sequences of the BP-1 peptides fused to different R5 peptides (R5′ corresponds to KRRIL) KRRIL-BP-1, BP-1-KRRIL, R5-BP-1, and BP-1-R5 (c); and membrane protein encapsulation in silica using BP-1-R5 constructs (d). New variants of the R5-membrane scaffold protein (MSP) fusion protein, shown in yellow and green, are used to create nanodiscs. Modified BP-1 peptides, shown in purple, can directly be used for the encapsulation of membrane proteins in silica.