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(a) The active site and accessory [FeS] cluster array in CPI [FeFe] hydrogenase (PDB 3C8Y). The reduction potential of each [FeS] cluster was determined and labeled. The proposed electron transfer array from distal [4Fe-4S] cluster to the active site was depicted favoring proton reduction. (b) [FeFe] Hydrogenase from Megasphaera elsdenii (MeHydA) and its N-terminal truncated form (MeH-HydA) which keeps the catalytic site but lacks the distal accessory [4Fe-4S] cluster.
