. 2021 Jul 24;5(8):nzab103. doi: 10.1093/cdn/nzab103

TABLE 2.

Prececal amino acid digestibility (%) in broiler chickens fed differently acidified diets with different calcium concentrations without (–) and with (+) supplementation of 1500 FTU phytase/kg¹

ABC	Ca con-centration	Phytase	Ala	Arg	Asx	Cys	Glx	Gly	His	Ile	Leu	Lys	Met	Phe	Pro	Ser	Thr	Tyr	Val
CaCO₃	low	–	80.4	87.0	77.5	68.5	85.4	73.8	79.2	81.6	81.6	82.3	87.8	82.6	77.4	76.6	73.7	80.4	79.8
		+	81.7	88.4	79.3	72.7	86.9	75.5	80.3	83.7	83.4	83.7	88.8	84.7	79.4	78.4	75.0	82.1	81.6
	high	–	76.0	84.9	74.1	66.3	82.9	70.9	75.9	78.1	78.0	79.1	84.6	79.1	74.9	73.0	69.6	76.9	76.4
		+	81.9	88.5	79.7	74.5	87.4	75.9	80.8	83.8	84.0	83.9	89.1	85.0	80.1	79.0	75.3	82.7	81.8
CaCO₃ + formic acid	low	–	81.4	87.6	78.0	68.7	86.0	74.5	79.9	82.4	82.6	83.0	88.7	83.5	78.4	77.6	74.5	81.3	80.8
		+	84.0	89.7	81.2	74.3	88.4	77.8	82.1	85.7	85.8	85.6	90.5	86.6	81.6	80.7	77.6	84.3	83.9
	high	–	77.6	86.1	75.2	67.5	83.6	72.0	77.4	79.0	79.1	80.4	85.4	80.1	76.1	74.2	71.2	77.8	77.4
		+	83.4	89.1	80.2	74.2	87.8	76.8	81.9	85.0	85.1	85.0	89.9	85.8	80.7	80.0	76.9	83.7	83.4
Ca-formate	low	–	81.4	87.6	78.1	68.7	86.1	75.0	79.4	83.0	82.6	83.0	88.7	83.5	78.4	77.4	74.7	81.6	81.3
		+	82.9	88.8	80.0	71.5	87.5	76.2	81.4	84.5	84.5	84.5	90.0	85.3	80.3	79.7	76.4	83.0	82.5
	high	–	78.4	85.9	75.1	67.5	84.1	72.0	77.2	80.1	80.4	80.6	86.8	81.0	76.8	74.5	71.7	78.5	78.4
		+	83.2	88.7	80.0	73.5	87.7	76.2	81.4	84.5	84.8	84.5	90.0	85.5	80.6	79.7	76.3	83.4	82.5
		SEM	0.88	0.40	0.59	0.91	0.48	0.63	0.70	0.69	0.77	0.63	0.77	0.67	0.70	0.71	0.84	0.75	0.73
Ca concentration × phytase²
	low	–	81.0^b	87.4^b	77.8^b	68.7^c	85.8^b	74.4^b	79.5^b	82.3^b	82.3^b	82.8^b	88.4^b	83.2^b	78.1^b	77.2^b	74.3^b	81.1^b	80.6^b
		+	82.8^a	89.0^a	80.2^a	72.8^b	87.6^a	76.5^a	81.3^a	84.6^a	84.6^a	84.6^a	89.8^a	85.5^a	80.4^a	79.6^a	76.3^a	83.2^a	82.7^a
	high	–	77.3^c	85.6^c	74.8^c	67.1^d	83.5^c	71.6^c	76.8^c	79.1^c	79.2^c	80.0^c	85.6^c	80.1^c	75.9^c	73.9^c	70.9^c	77.7^c	77.4^c
		+	82.8^a	88.8^a	80.0^a	74.0^a	87.6^a	76.3^a	81.4^a	84.4^ab	84.6^a	84.5^a	89.7^a	85.5^a	80.5^a	79.6^a	76.2^a	83.2^a	82.5^a
		SEM	0.68	0.27	0.40	0.64	0.32	0.44	0.52	0.49	0.58	0.48	0.59	0.48	0.51	0.50	0.63	0.55	0.53
ABC³
CaCO₃			80.0^B	87.2^B	77.6^B	70.5	85.7^B	74.0^B	79.1^B	81.8^A	81.8^B	82.3^B	87.6^B	82.8^B	77.9^B	76.7^B	73.4^B	80.5^B	79.9^B
CaCO₃ + formic acid			81.6^A	88.1^A	78.7^A	71.2	86.5^A	75.2^A	80.3^A	83.0^B	83.1^A	83.5^A	88.6^A	84.0^A	79.2^A	78.1^A	75.0^A	81.8^A	81.3^A
Ca-formate			81.5^A	87.7^A	78.3^AB	70.3	86.4^A	74.8^A	79.8^AB	83.0^B	83.1^A	83.1^A	88.9^A	83.8^A	79.0^A	77.8^A	74.8^A	81.6^A	81.2^A
SEM			0.65	0.25	0.37	0.60	0.29	0.42	0.50	0.46	0.55	0.46	0.56	0.45	0.48	0.47	0.59	0.52	0.50
ANOVA
ABC			0.002	0.003	0.029	0.24	0.027	0.011	0.010	0.007	0.004	0.003	0.011	0.013	0.008	0.006	0.002	0.015	0.002
Ca concentration			<0.001	<0.001	0.723	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	0.003	<0.001	<0.001	<0.001	<0.001	<0.001
Phytase			<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
ABC × Ca concentration			0.60	0.93	0.82	0.64	0.60	0.83	0.95	0.75	0.43	0.84	0.53	0.56	0.45	0.66	0.88	0.72	0.83
ABC × phytase			0.54	0.47	0.63	0.20	0.49	0.24	0.91	0.12	0.29	0.49	0.60	0.29	0.41	0.72	0.44	0.38	0.11
Ca concentration × phytase			<0.001	<0.001	0.003	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	0.003	<0.001	<0.001	<0.001	<0.001	<0.001
ABC × Ca concentration × phytase			0.71	0.44	0.44	0.40	0.57	0.46	0.53	0.86	0.64	0.65	0.68	0.69	0.63	0.66	0.64	0.78	0.91

Values are least square means, n = 6 pens/treatment with 15 birds/pen. Labeled means of the Ca concentration × phytase interaction in a column without a common lowercase superscript letter differ significantly, P ≤ 0.050; labeled means of the ABC effect in a column without a common uppercase superscript letter differ significantly, P ≤ 0.050; ABC, acid-binding capacity; Ala, alanine; Arg, arginine; Asx, aspartic acid/asparagine; Ca, calcium; CaCO₃, calcium carbonate; Cys, cysteine; FTU, phytase units; Glx, glutamic acid/glutamine; Gly, glycine; His, histidine; Ile, isoleucine; Leu, leucine; Lys, lysine; Met, methionine; Phe, phenylalanine; Pro, proline; Ser, serine; Thr, threonine; Tyr, tyrosine; Val, valine.

Other 2-way interaction means not presented because not significant (P ≥ 0.11).

Other main effect means not presented because of the significant interaction (P ≤ 0.003) between Ca concentration and phytase.