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. 2021 Aug 9;11:672724. doi: 10.3389/fonc.2021.672724

Figure 4.

Figure 4

CircFNDC3B enhances SLC7A11 expression by targeting miR-520d-5p. (A) The interaction of miR-520d-5p with circFNDC3B and SLC7A11 was identified in ENCORI online database. (B–D) MiR-520d-5p mimic or control mimic was used to treat CAL27 and SCC15 cells. (B) The RT-qPCR analysis of miR-520d-5p expression. (C, D) Dual luciferase reporter assays of luciferase activities of circFNDC3B and SLC7A11 mRNA 3’UTR, respectively. (E) The RT-qPCR analysis of miR-520d-5p expression in CAL27 and SCC15 cells treated with circFNDC3B shRNA. (F) The RT-qPCR analysis of SLC7A11 expression in CAL27 and SCC15 cells treated with miR-520d-5p mimic. (G) Western blot analysis of SLC7A11 expression in CAL27 and SCC15 cells treated with circFNDC3B shRNA and miR-520d-5p inhibitor. N = 6, mean ± SEM: ns no significance, **P < 0.01. (H) The expression of circFNDC3B, SLC7A11, and miR-520d-5p was analyzed by qPCR in the clinical OSCC tissues and the related non-tumor tissues. (I) The correlation of circFNDC3B, SLC7A11, and miR-520d-5p was analyzed by qPCR in the clinical OSCC tissues. mean ± SEM: **P < 0.01.