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. 2021 Aug 16;10:e68484. doi: 10.7554/eLife.68484

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© 2021, Victorino et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Representative histograms (top) and quantification (bottom) of CD69 MFI on NK cells and frequency of KLRG1⁺ and IFNγ⁺ NK cells at day 1.5 post-infection (pi) of 1αKO or FL control mice. Data are from 2–3 independent experiments with 4–6 mice per group. (B) Representative histograms (top) and quantification (bottom) of CD69 and Granzyme B MFI (GrzmB) of NK cells, and frequency of KLRG1⁺ NK cells at day 3 pi. Data are from two independent experiments with four mice per group. (C) 1αKO or FL control mice were injected with PBS or Poly (I:C) then 3 days later 20 × 10⁶ splenocytes at 1:1 ratio of CFSE-labeled MHCI-sufficient (CFSE^High) and -deficient (CFSE^Low) were intravenously transferred into mice. MHC-deficient splenocyte elimination was measured 3 hr post-transfer by flow cytometry. Data are from three independent experiments with 3–4 mice per group. Data depict mean ± SEM, with each data set containing data indicated number of mice per group from independent experiments. Unpaired t-test was performed on (A, B) or one-way ANOVA (C). Statistical significance indicated by n.s., no significant difference; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Figure 3—figure supplement 1. — (A) Representative histograms (top) and quantification (bottom) of CD69 MFI on NK cells and frequency of KLRG1⁺ and IFNγ⁺ NK cells at day 1.5 post-infection (pi) of 1αKO or FL control mice. Data are from 2–3 independent experiments with 4–6 mice per group. (B) Representative histograms (top) and quantification (bottom) of CD69 and Granzyme B MFI (GrzmB) of NK cells, and frequency of KLRG1⁺ NK cells at day 3 pi. Data are from two independent experiments with four mice per group. (C) 1αKO or FL control mice were injected with PBS or Poly (I:C) then 3 days later 20 × 10⁶ splenocytes at 1:1 ratio of CFSE-labeled MHCI-sufficient (CFSE^High) and -deficient (CFSE^Low) were intravenously transferred into mice. MHC-deficient splenocyte elimination was measured 3 hr post-transfer by flow cytometry. Data are from three independent experiments with 3–4 mice per group. Data depict mean ± SEM, with each data set containing data indicated number of mice per group from independent experiments. Unpaired t-test was performed on (A, B) or one-way ANOVA (C). Statistical significance indicated by n.s., no significant difference; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.