KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-SCN2A (NaV1.2) | Alomone Labs | Cat#ASC-002; RRID: AB_2040005 |
| GAPDH (D16H11)XP® Rabbit mAb | Cell Signaling Technology | Cat#5174S; RRID: RRID: AB_10622025 |
| β-Actin (8H10D10) Mouse mAb | Cell Signaling Technology | Cat#3700S; RRID: RRID: AB_2242334 |
| IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody | LI-COR Biosciences | P/N: 926–68071; RRID: AB_10956166 |
| IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody | LI-COR Biosciences | P/N: 926–68070; RRID: AB_10956588 |
| IRDye® 680RD Goat anti-Rat IgG Secondary Antibody | LI-COR Biosciences | P/N: 926–68076; RRID: AB_10956590 |
| Bacterial and virus strains | ||
| pAAV-EF1a-mCherry-IRES-Flpo | Fenno et al., 2014 | Addgene 55634-AAVrg |
| PHP.eB-EF1a-mCherry-IRES-Flpo-WPRE-hGH | This paper; Penn Vector Core | N/A |
| PHP.eB-Ef1a-DO-mCherry-WPRE-pA | This paper; Bio-Detail Corporation | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| NEUROBIOTIN Tracer | Vector Laboratories | SP-1120 |
| Streptavidin, Alexa Fluor 488 Conjugate | Thermo Fisher Scientific | S32354 |
| 4% paraformaldehyde | Thermo Fisher Scientific | AAJ19943K2 |
| TRIzol Reagent | Thermo Fisher Scientific | 15596018 |
| RIPA Lysis and Extraction Buffer | Thermo Fisher Scientific | 89901 |
| Protease Inhibitor Mini Tablets | Thermo Fisher Scientific | A32953 |
| Pierce Rapid Gold BCA Protein Assay Kit | Thermo Fisher Scientific | A53226 |
| NuPAGE LDS Sample Buffer (4 ×) | Thermo Fisher Scientific | NP0007 |
| NuPAGE Sample Reducing Agent (10 ×) | Thermo Fisher Scientific | NP0009 |
| PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa | Thermo Fisher Scientific | 26619 |
| PowerUp SYBR Green Master Mix | Thermo Fisher Scientific | A25777 |
| SYBR Safe DNA Gel Stain | Invitrogen by Thermo Fisher Scientific | S33102 |
| Tween 20 | Fisher Scientific | AAJ20605AP |
| Formaldehyde solution | Sigma-Aldrich | F8775 |
| Glutaraldehyde solution | Sigma-Aldrich | 354400 |
| 4-(Trifluoromethyl)-L-phenylglycine | Sigma-Aldrich | 16599 |
| Choline chloride | Sigma-Aldrich | C7527 |
| Bovine Serum Albumin | Sigma-Aldrich | A9418 |
| 1 kb DNA Ladder | Sigma-Aldrich | D0428–1VL |
| Agarose | Sigma-Aldrich | A9539 |
| Tetrodotoxin citrate | Abcam | ab120055 |
| CGP 55845 hydrochloride | Abcam | ab120337 |
| D-AP5 | Abcam | ab120003 |
| CNQX disodium salt | Abcam | ab120044 |
| ZD7288 | Abcam | ab120102 |
| Pimaric acid | MP Biomedicals | 219895105 |
| (2-Fluorophenyl) glycine | VWR | 101795–980 |
| Odyssey® Blocking Buffer (PBS) | LI-COR Biosciences | 927–40000 |
| Intercept® T20 (TBS) Antibody Diluent | LI-COR Biosciences | 927–65001 |
| 10 × Tris Buffered Saline | Bio-Rad | 1706435 |
| 10 × Tris/Glycine Buffer | Bio-Rad | 1610771 |
| 10 × Tris/Glycine/SDS Electrophoresis Buffer | Bio-Rad | 1610772 |
| Immobilon®-FL PVDF membrane | Millipore | IPFL00010 |
| 50 × pH 8,3 tris-acetate EDTA buffer | Millipore | 1.06174 |
| α-Dendrotoxin | Alomone Labs | D-350 |
| Tetrodotoxin citrate | Alomone Labs | T-550 |
| Picrotoxin | Alomone Labs | P-325 |
| Optimal Cutting Temperature compound | Tissue Tek | 4583 |
| NucleoSpin® DNA RapidLyse | Macherey-Nagel | 740100.250 |
| Liquid Proteinase K | Macherey-Nagel | 740396 |
| Critical commercial assays | ||
| β-Gal Staining Set | Sigma-Aldrich | 11828673001 |
| Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase | Thermo Fisher Scientific | K1672 |
| Deposited data | ||
| Raw and processed RNA sequencing data | This paper | GEO: GSE179818 |
| Custom scripts | This paper | Zenodo Repository https://zenodo.org/record/5098353 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6N-Scn2a1tm1aNarl/Narl | The National Laboratory Animal Center Rodent Model Resource Center | Scn2a WT/gt |
| Oligonucleotides | ||
| See Table S1 | N/A | |
| Recombinant DNA | ||
| pAAV-EF1a-mCherry-IRES-Flpo | Fenno et al., 2014 | Addgene Plasmid # 55634 |
| pUCmini-iCAP-PHP.eB | Chan et al., 2017 | Addgene plasmid # 103005 |
| pAAV-Ef1a-DO-mCherry-WPRE-pA | Saunders et al., 2012 | Addgene plasmid # 37119 |
| Software and algorithms | ||
| ImageJ (Fiji) | Eliceiri/LOCI group | https://fiji.sc/ |
| Image Studio Lite 5.2 | LI-COR Biosciences | https://www.licor.com/bio/image-studio-lite/d5 |
| pClamp 11.1 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system |
| OriginPro version 2020 | OriginLab Corporation | https://www.originlab.com/ |
| GraphPad Prism version 8.0.0 for Windows | GraphPad Software | https://www.graphpad.com/ |
| Adobe Illustrator 2020 | Adobe | https://www.adobe.com/ |
| Other | ||
| Odyssey® CLx Imaging System | LI-COR Biosciences | https://www.licor.com/bio/odyssey-clx/ |
| NanoDrop OneC Microvolume UV-Vis Spectrophotometer | Thermo Fisher Scientific | N/A |
| C1000 Touch PCR thermal cycler | Bio-Rad | N/A |
| MultiPhoton confocal microscopy | Nikon | A1R-MP |
| LSM 900 scanning confocal microscope | Zeiss | N/A |
| Cryostat | Leica Biosystems | CM1950 |
| Vibrating blade microtome | Leica Biosystems | VT1200 S |
| Micropipette Puller | Sutter Instrument | P-1000 |
| Borosilicate glass with filament | Sutter Instrument | BF150-110-10 |
| Axon MultiClamp 700B Microelectrode Amplifier | Molecular Devices | N/A |
| Axon Digidata 1550B Plus HumSilencer | Molecular Devices | Digidata 1550B4 |
| BX-51WI microscope | Olympus | N/A |
| IR-2000 Infrared Monochrome Video Camera | Dage-MTI | N/A |
| Stereotaxic for Mouse, Dual M, Digital, with Adaptor (68055) | RWD Life Science Inc. | 68046 |