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. Author manuscript; available in PMC: 2021 Aug 23.
Published in final edited form as: Nat Neurosci. 2021 Feb 8;24(3):343–354. doi: 10.1038/s41593-020-00796-z

Extended Data Fig. 10. Characterization of the APPSWE+/+ and WT isogenic lines.

Extended Data Fig. 10

A) Sanger sequencing shows that the APPSWE+/+ line is homozygous for the GA>TC mutation as compared to the isogenic wildtype line. B) IF shows that both lines express the pluripotency markers of SOX2, NANOG, and OCT4. Scale bar = 100μM. C) Quantification of amyloid peptides 38, 40, and 42 in neuronal cultures shows that APPSWE+/+ neurons have higher levels of all peptides as compared to isogenic WT neurons. n=3 cell culture supernatants. D) Increased C1Q secretion by ELISA in APPSWE+/+ tri-cultures, ****p<0.0001, two-way ANOVA with Sidak’s post hoc test, n = 3 cell culture supernatants, error bars = SD, center = mean. E) Increased C1Q secretion in C3KOM but not C3KOA tri-cultures. n=3 cell culture supernatants, error bars = SD, center = mean.