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. 2021 Aug 14;2021:4504363. doi: 10.1155/2021/4504363

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Copyright © 2021 Zhongkuan Lyu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of LPS and Gly interventions on expression levels of pyroptosis-related proteins and observations of pyroptosis locations. (a, b) Expression levels of pro-/cleaved caspase-11 and GSDMD-FL/N by Western blotting analysis, n = 6. (c) Representative pictures of the double immunofluorescence staining (white arrows) of caspase-11 (red) colocalized with Iba-1 or GFAP (green), respectively, scale bars, 50 μm. (d, e) Expression levels of NLRP3, cleaved caspase-1, IL-6, and cleaved IL-1β, n = 6. Data are presented as mean ± SD. ^##P < 0.01, I/R group versus sham group; ^▲P < 0.05, ^▲▲P < 0.01, I/R + LPS group versus I/R group; ^∗∗P < 0.01, I/R + Gly group versus I/R group.