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. 2021 Aug 14;2021:4504363. doi: 10.1155/2021/4504363

Figure 5.

Figure 5

Influences of pyroptosis on AQP-4 polarization and Aβ clearance at 24 h after reperfusion. (a) Representative pictures of immunohistochemical staining of AQP-4. White arrows represent normal AQP-4 polarization, and black arrows represent the loss of AQP-4 polarization with obvious dispersion and perturbed expression, scale bars, 50/20 μm. (b) Representative pictures of double immunofluorescence staining of Aβ (red) colocalized with GFAP (green), scale bars, 50 μm. (c, d) Protein levels of AQP-4 by Western blotting analysis, n = 6. (e, f) Protein levels of Aβ1-42 oligomer by Western blotting analysis, n = 6. Data are presented as mean ± SD. ##P < 0.01, I/R group versus sham group; ▲▲P < 0.01, I/R + LPS group versus I/R group; ∗∗P < 0.01, I/R + Gly group versus I/R group.