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. 2021 May 27;476(10):3623–3633. doi: 10.1007/s11010-021-04186-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — SPRY1 was a downstream target of miR-23b. A The relationship between SPRY1 and miR-23b was predicted by DianaTools. The interaction between miR-23b and SPRY1 was further validated by B dual-luciferase reporter assay and C, D pull-down assay. E The expression of SPRY1 at different time points post treatment of IL-1β in chondrocytes was detected by Western blot. F The expression of SPRY1 in chondrocytes with miR-23b overexpression or inhibition was examined using Western blot. *P < 0.05