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. 2021 Aug 23;11:17075. doi: 10.1038/s41598-021-96567-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of yield and RNA integrity number (RIN) of RNA isolated from whole blood samples. (a,b) Comparison of (a) yield and (b) RIN of RNA among protocols P-37, P-Al, N-37, and N-Al. Data are shown as mean ± standard deviation (n = 7). *p < 0.05 (two-way analysis of variance) for (a) NucleoSpin versus PAXgene, or (b) thawing in a water bath versus thawing on an aluminum block. (c,d) Comparison of (c) yield and (d) RIN of RNA among protocols N-37, N-Al, and N-Ice. *p < 0.05 (repeated-measures analysis of variance with Bonferroni correction) for N-37 versus N-Al. n.s. not significant. The images were created using EZR software version 1.54 (http://www.jichi.ac.jp/saitama-sct/SaitamaHP.files/statmedEN.html), Microsoft Excel 2016 MSO (16.0.14131.20278) 32 bit (https://www.microsoft.com/) and Adobe Illustrator CS5 (https://www.adobe.com/).