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. 2021 Aug 23;11:17075. doi: 10.1038/s41598-021-96567-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of GAPDH and RPS18 expression in RNA isolated from whole blood samples using six different protocols. (a,b) Data are shown for each sample as mean ± standard deviation (n = 7). (a) *p < 0.05 for P-Con versus P-37 and P-37 versus P-Al (repeated-measures analysis of variance with Bonferroni correction). (b) *p < 0.05 for P-Con versus P-37, P-37 versus N-37, and P-37 versus N-Al (repeated-measures analysis of variance with Bonferroni correction). GAPDH glyceraldehyde 3-phosphate dehydrogenase, RPS18 ribosomal protein S18. The images were created using EZR software version 1.54 (http://www.jichi.ac.jp/saitama-sct/SaitamaHP.files/statmedEN.html), Microsoft Excel 2016 MSO (16.0.14131.20278) 32 bit (https://www.microsoft.com/) and Adobe Illustrator CS5 (https://www.adobe.com/).