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. 2021 Aug 23;11:17075. doi: 10.1038/s41598-021-96567-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Procedure for thawing frozen whole blood samples stored in ethylenediaminetetraacetic acid (EDTA) tubes. (a) Wrap aluminum foil tightly around the EDTA tube. (b) Repeat the wrapping process until the aluminum foil does not lose its shape when the EDTA tube is pulled out. (c) Beat the top and bottom of the aluminum with a hammer. (d) Beat the side of the aluminum with a hammer. (e) Repeat steps (a–d) until the thickness of the aluminum block is around 1 cm. (f) Place the EDTA tube containing the frozen whole blood sample in an aluminum block, sandwich it between aluminum racks for microtubes, and blow it with a fan. The images were created using Adobe Illustrator CS5 (https://www.adobe.com/).