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. 2021 Aug 23;11:17077. doi: 10.1038/s41598-021-96449-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Translocation of CD24 from cytosol to membrane is observed in patient-derived breast cancer cells under drug stress. (a) Localization of CD24 in MDA-MB-231, MACL-1 and MGSO-3 cells after staining with anti-CD24/Pe-Cy7 (extracellular and intracellular staining). Dot-plots are representative of triplicates. (b) Frequency of CD24⁺ cells according to the detection of CD24 in membrane (extracellular) or in cytosol (intracellular). (c) CD24⁺ cell enrichment in breast cancer cell lines during Dx treatment. MDA-MB-231, MACL-1 and MGSO-3 cells were treated with Dx (0.6 μM) according to the indicated times and stained using anti-CD24/FITC. The dot-plots are representative of triplicates. (d) Frequency of CD24⁺ cells under Dx treatment according to the detection of CD24 in membrane. Data were plotted as means of triplicates ± SD. ***p < 0.001, **p < 0.01, *p < 0.05 (one-way ANOVA with bonferroni post-test).