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. 2021 Aug 23;11:17069. doi: 10.1038/s41598-021-96627-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pseudoacetylation at KXGS motifs do not lead to significant aggregation even with K18 seeds. HEK293T cells were transfected with 0N4R WT tau and 0N4R tau acetylmimetics K259Q, K290Q, K321Q, and K353Q. Cell lysates were fractionated into Triton soluble and insoluble fractions to isolate potential tau aggregates. Immunoblots were probed with tau antibody 3026. (A) WT tau does not aggregate with or without K18 seeds. (B) K259Q, (C) K290Q, (D) K321Q, and (E) K353Q 0N4R tau are also resistant to aggregation with or without K18 seeding. (F) P301L 0N4R tau was used as a positive control. The relative molecular weight markers are shown on the left.