Fig. 1. SLC26A2 was overexpressed in PDGF-stimulated HASMCs, and SLC26A2 knockdown restrained cell proliferation and migration. (A) Volcano plot of differently expressed genes in the GEO database. Red dots indicate highly expressed genes and green dots represent poorly expressed genes. (B) The expression of SLC26A2 in samples with acute asthma (n=56) and healthy samples (n=50). (C) Western blotting showed SLC26A2 expression in PDGF-induced HASMCs. (D) Western blotting showed SLC26A2 expression in PFGF-induced HASMCs transfected with siSLC26A2. (E and F) The effect of SLC26A2 on the proliferation of PDGF-induced HASMC was assessed by MTT and EdU analyses. (G) The effect of SLC26A2 on the migration of PDGF-induced HASMC was evaluated by Transwell analysis (scale bar: 100 µm). (H) Western blotting showed PCNA and MMP-9 expressions in PDGF-induced HASMCs transfected with siSLC26A2. Data are presented as the mean±SD of three independent experiments. ^*P<0.05, compared to scrambled control group, ^†P<0.05, compared to PDGF+si-NC group. HASMCs, human airway smooth muscle cells; PGDF, platelet-derived growth factor; NC, negative control.