Fig. 2. SLC26A2 knockdown enhanced contractile phenotype marker protein expression and restrained inflammatory factor production in PDGF-stimulated HASMCs. (A) Immunofluorescent staining of α-SMA (green) in HASMCs (scale bar: 50 µm). (B) Western blotting showed α-SMA and Calponin expressions in HASMC. ELISA assays of (C) IL-4, (D) IL-6, and (E) IL-13 levels in HASMC culture supernatant. Data are presented as the mean±SD of three independent experiments. *P<0.05, compared to scrambled control group, †P<0.05, compared to PDGF+si-NC group. α-SMA, α-smooth muscle actin; PGDF, platelet-derived growth factor; HASMCs, human airway smooth muscle cells; IL, interleukin; NC, negative control.
