Fig. 4. Knockdown of miR-9-5p significantly reversed the effect of SLC26A2 knockdown on PDGF-stimulated HASMCs. (A and B) The proliferation of HASMC was evaluated by MTT and EdU analyses. (C) The migration of HASMC was evaluated by Transwell analysis (scale bar: 100 µm). (D) Western blotting showed related proteins in HASMC. (E) Immunofluorescent staining of α-SMA (green) in HASMCs (scale bar: 50 µm). (F) ELISA assays of IL-4, IL-6, and IL-13 levels in HASMC culture supernatant. Data are presented as the mean±SD of three independent experiments. ^*p<0.05, compared to scrambled control group, ^†P<0.05, compared to PDGF+si-NC group, ^‡P<0.05, compared to PDGF+si-SLC26A2 group. HASMCs, human airway smooth muscle cells; PGDF, platelet-derived growth factor; α-SMA, α-smooth muscle actin; IL, interleukin; NC, negative control.