Fig. 6. Knockdown of NEAT1 regulated miR-9-5p/SLC26A2 to inhibit the effect of PDGF on HASMCs. (A) qRT-PCR showed NEAT1 level in cells. (B and C) The proliferation of HASMC was evaluated by MTT and EdU analyses. (D) The migration of HASMC was evaluated by Transwell analysis (scale bar: 100 µm). (E) Western blotting showed related proteins in HASMC. (F) Immunofluorescent staining of α-SMA (green) in HASMCs (scale bar: 50 µm). (G) ELISA assays of IL-4, IL-6, and IL-13 levels in HASMC culture supernatant. Data are presented as the mean±SD of three independent experiments. *p<0.05, compared to si-NC or scrambled control group, †p<0.05, compared to PDGF+si-NC group. HASMCs, human airway smooth muscle cells; PGDF, platelet-derived growth factor; α-SMA, α-smooth muscle actin; IL, interleukin; NC, negative control.
