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. 2021 Aug 23;9:38. doi: 10.1038/s41413-021-00153-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Deletion of Glut1 in chondrocytes decreases cellularity, disrupts cellular homeostasis, and ultimately causes cartilage fibrosis in AC. a Safranin O/Fast Green staining of knee sections of control and Glut1 LOF mice at 4 months and 7 months. N = 6. Scale bar, 100 μm. b Histomorphometric analyses of chondrocyte numbers in knee sections at 4 months. The data are the mean ± SD. N = 6. *P < 0.05. c RT-qPCR analyses for Glut1, Col2a1, Acan, Adamts5 and Mmp13 in control and Glut1 KO primary AC chondrocytes. All mRNA abundances were normalized to that of β-actin and then normalized to those of the controls. The data are the mean ± SD. N = 3. *P < 0.05 relative to the controls. d Immunostaining for Mmp13 and Col3a1 in knee sections of control and Glut1 LOF mice at 4 months (N = 6) or of Col3a1 in biopsies of normal and osteoarthritic cartilage from human patients (N = 3). Scale bar, 100 μm. e Safranin O/Fast Green staining and Col3a1 immunostaining of knee sections of control and Glut1 LOF mice at 4 weeks following MLI injury. N = 6. Scale bar, 100 μm. f OARSI scores for the medial tibial plateau and femoral condyle at 4 weeks following MLI injury. The data are the mean ± SD. N = 6. *P < 0.05 compared to the controls