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. 2021 Aug 23;11:17029. doi: 10.1038/s41598-021-96227-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dose-dependent effect of selected compounds on p-Tau in human neurons and in an in vitro GSK3β assay. (A) 20 h treatment with CHIR-99021, enzastaurin and ruboxistaurin dose-dependently decreased mean PHF-1 intensity in both cell bodies and neuronal processes in both control and patient iNgn2 neurons. Mean ± SEM of two biological replicates; dots represents individual values. One-way ANOVA, Dunnett’s post hoc test, ns = not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to DMSO. (B) IC₅₀ values of CHIR-99021 (positive control, n = 2), enzastaurin (n = 4) and ruboxistaurin (n = 4) as determined in the ADP-Glo kinase assay for GSK3β, where n is the number of replicate wells.