Figure 1.

Step by step differentiation and maturation to generate functional human islet-like organoids. By using chemically defined recombinant proteins and small molecules, hPSCs are introduced pancreatic lineage specification. Islet-like organoids contains insulin producing β-cells, glucagon producing α-cells, somatostatin producing δ-cells, and pancreatic polypeptide producing γ-cells. The representative duration and small compounds/recombinant proteins used for β-cells differentiation are listed in the Figure.