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. 2021 Aug 10;12:658755. doi: 10.3389/fpls.2021.658755

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Copyright © 2021 Shiraku, Magwanga, Cai, Kirungu, Xu, Mehari, Hou, Wang, Agong, Peng, Wang, Zhou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic observation of leaf and seed pods and physiological analysis. (A) Representative leaf image of WT and overexpressed lines before and after drought and salt treatment. (B) Representative image of the seed pod of WT and overexpressed lines before and after drought and salt treatment. (C) DAB staining assay of WT and GhACX3-overexpressed lines in untreated, drought, and salt stress plants. (D) Quantitative determination of (i) chlorophyll content, (ii) ion leakage, (iii) excised leaf water loss, and (iv) relative leaf water content. Different letters indicate significant differences between wild-type and VIGS plants (ANOVA; p < 0.05). Untreated indicates normal conditions.