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. 2021 Aug 10;12:658755. doi: 10.3389/fpls.2021.658755

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Copyright © 2021 Shiraku, Magwanga, Cai, Kirungu, Xu, Mehari, Hou, Wang, Agong, Peng, Wang, Zhou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Root germination and elongation assay in Arabidopsis plants. (A) Analysis of germination in GhACX3-overexpressing lines and wild-type under stress conditions. (B) Phenotypic comparison of seedlings grown on 50% MS medium or MS with 100 mM of mannitol, 300 mM of mannitol, 75 mM of NaCl, and 150 mM of NaCl after 8 days. (C,D) The germination rate of the seedlings grown on MS medium and different concentrations of mannitol and NaCl. Analysis of root elongation in GhACX3-overexpressing lines and wild-type under MS added NaCl and mannitol and statistical analysis of the root lengths. Different letters above the columns indicate significant differences at P < 0.05. MS represent normal conditions.