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. 2021 Aug 10;12:658755. doi: 10.3389/fpls.2021.658755

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Copyright © 2021 Shiraku, Magwanga, Cai, Kirungu, Xu, Mehari, Hou, Wang, Agong, Peng, Wang, Zhou and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization and confirmation of silencing. (A) Subcellular localization in G. hirsutum, G. arboretum, and G. raimondii as predicted by WoLF PSORT. (B) Subcellular localization of GhACX3. GhACX3-GFP represents the gene and GFP represents the control that is an empty vector. (C) Confirmation of silencing. (i) Representative images of PDS (ii) expression of the silenced gene in the leaf, stem, and root. (iii) Relative expression of the GhACX3 gene in normal condition (WT), empty vector (TRV2: 00), and silenced GhACX3 plants.