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. 2021 May 22;38(9):3789–3803. doi: 10.1093/molbev/msab157

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Oligomeric state and superoxide dismutase activity of RbCuZnSOD and BbCuZnSOD. (A) SEC chromatograms showing the increase in RbZnSOD hydrodynamic radius as concentration increases. (B) SEC chromatograms showing distinct RbCuZnSOD monomers and dimers along with BbCuZnSOD1 and SOD1dimers. (C) SDS-PAGE showing RbCuZnSOD dimers are resistant to heating and reduction in contrast to BbCuZnSOD. D—dimer, M—monomer, BME—β-mercaptoethanol. (D) Reducing and denaturing SDS-PAGE (left) of CuZnSODs assessed for superoxide dismutase activity by in-gel nitrotetrazolium blue staining (Beauchamp and Fridovich 1971). RbCuZnSOD retains activity in the monomeric state.