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. 2021 May 22;38(9):3789–3803. doi: 10.1093/molbev/msab157

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Intrasubunit disulfide bond formation and homodimerization. (A) Reducing and denaturing SDS-PAGE showing AMS-conjugated CuZnSODs following incubation over a time course. BbZnSOD is unreactive to AMS after 21 h showing free protein thiols have been sequestered indicating intrasubunit disulfide formation. In contrast 2 weeks is necessary for human SOD1 and RbZnSOD to display significant disulfide formation. (B) SEC chromatograms showing BbZnSOD homodimerization follows the same course as intrasubunit disulfide formation. Human SOD1 does eventually form homodimers while RbZnSOD remains monomeric over the same time period.