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. 2021 Aug 9;24(9):102963. doi: 10.1016/j.isci.2021.102963

Figure 3.

Figure 3

Digestion and absorption of MCT oil in gastrointestinal tract

(A) Intestinal lipase activity in fasted 15-week-old male WT mice (n = 3 mice). Sto, Duo, Jej, and Ile indicate the stomach, duodenum, jejunum, and ileum, respectively. Data indicate the mean ± SEM.

(B) Lipid analysis of intestinal contents by thin-layer chromatography at 30 min after loading of olive oil or MCT oil in 15-week-old male WT mice. Std indicates standard lipid. Sto, Duo, Jej, and Ile indicate the contents in the stomach, duodenum, jejunum, and ileum, respectively. TG, FA, DG, and MG indicate triglyceride, fatty acid, diglyceride, and monoglyceride, respectively. The test was performed three times, and representative data from each of them are presented.

(C) Plasma fatty acid composition in portal vein (PV) in the fasted state and at 30 min after ingestion of saline, olive oil, or MCT oil (n = 4 mice). Orange bars, yellow bars, and gray bars indicate caprylic acid (C8:0), capric acid (C10:0), and LCFAs, respectively. PV plasma samples from all mice are presented, and 1 to 4 indicate the sample numbers.

(D) Plasma fatty acid composition in PV and inferior vena cava (IVC) at 30 min after infusion of MCT oil into the stomach following stomach ligation (n = 3 mice).

(E and F) Plasma fatty acid composition in PV and inferior vena cava (IVC) at 30 min after infusion of MCT oil or MCT oil + lipase inhibitor into the intestine following intestine ligation (n = 3 mice). Red bars indicate medium-chain fatty acids (MCFAs), and gray bars indicate long-chain fatty acids (LCFAs). Plasma samples from all mice are presented, and 1 to 3 indicate the sample numbers.

(G and H)(G) Intestinal lipase activity and (H) remaining bile volume in the gallbladder at 30 min after ingestion of saline, olive oil, or MCT oil (n = 6 mice). White circles, gray circles, and black circles indicate saline, olive oil, and MCT oil, respectively. Data indicate the mean ± SEM. Statistical significance was calculated by One-way ANOVA with Tukey’s test using SPSS statistics. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. n.s. no significance.