REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
Olive oil | Nisshin OilliO Group | N/A |
MCT oil | Nisshin OilliO Group | N/A |
[Thr28, Nle31]-Cholecystokinin (25-33) | AnaSpec | Cat# AS-22944 |
Proglumide | Toronto Research Chemicals | Cat# P755955 |
Proglumide sodium salt solid | Sigma Aldrich | Cat# M006 |
Orlistat | Tocris Bioscience | Cat# 3540/50 |
Linolenic acid | Sigma Aldrich | Cat# L2376 |
Oleic acid | Sigma Aldrich | Cat# O1008 |
Octanoic acid (Caplyric acid) | Sigma Aldrich | Cat# O3907 |
Decanoic acid (Capric acid) | Sigma Aldrich | Cat# C1875 |
Critical commercial assays | ||
LBIS Mouse Insulin ELISA kit | FUJIFILM Wako Shibayagi Corporation | Cat# AKRIN-011T; RRID: AB_2810836 |
Rat/Mouse GIP (total) ELISA | Merck | Cat# EZRMGIP-55K; RRID: AB_2801384 |
VPLEX GLP-1 Total Kit | Meso Scale Discovery | Cat# K1503PD-1 |
Lipase kit S | SB Bioscience | Cat# 4987-116-88021-3 |
Bio-Ras Protein Assay Dye Reagent Concentrate | Bio-Rad | Cat# 5000006JA |
FLIPR Calcium 4 Assay Kit | Molecular Devices | Cat# R8141; RRID: AB_11195743 |
RNeasy Mini Kit | Qiagen | Cat# 74004 |
PrimeScript RT reagent kit | Takara Bio | Cat# RR037B |
SYBR Green PCR Master Mix | Applied Biosystems | Cat# 4334973 |
Experimental models: Cell lines | ||
Mouse: STC-1 cell line | Laboratory of Akira Hirasawa Tanaka et al. (2008) |
RRID:CVCL_J405 |
Human: T-REx GPR40 cell line | Laboratory of Akira Hirasawa Hara et al. (2009) |
N/A |
Human: T-REx GPR120 cell line | Laboratory of Akira Hirasawa Ichimura et al. (2012) |
N/A |
Experimental models: Organisms/strains | ||
C57BL/6J mice | Japan SLC | N/A |
GIP KO mice | Nasteska et al. (2014) | N/A |
Oligonucleotides | ||
Silencer®Select Ffar4 Mouse | Invitrogen | Cat# s200889 |
Silencer®Select Ffar1 Mouse | Invitrogen | Cat# s107454 |
Silencer™Select Negative Control No. 1 siRNA | Invitrogen | Cat# 4390844 |
Primers for quantitative real-time PCR | Sankoda et al. (2017) | N/A |
Software and algorithms | ||
Igor Pro | WaveMetrics | RRID:SCR_000325 http://www.wavemetrics.com/products/igorpro/igorpro.htm |
Molegro Virtual Docker software | Molegro ApS | RRID:SCR_000190 http://molegro-virtual-docker.software.informer.com/ |
La Theta (LCT-100M) | Hitachi Aloka Medical | N/A |
SPSS Statistics | IBM | RRID:SCR_019096 https://www.ibm.com/products/spss-statistics |
Other | ||
TLC Silica gel 60 F254 25 Aluminium sheets 20 x 20 cm | Merck | Cat# 105554 |
Rodent Diet With 45 kcal% Fat | Reserch Diet Inc. | Cat# D12451 |