Figure 5.
YAP deletion increases expression of AT2 cell signature genes
RNA-seq analyses were performed on EPCAM+ cells isolated from PND14 YAPdeleted (N = 5) and WT (N = 4) mice.
(A) Volcano plot of the differentially expressed genes (p < .05, FC > 1.5) shows increase in AT2 markers Abca3, Sftpb and Sftpa1, and decrease in known YAP targets Ajuba, Axl and Yap itself.
(B) Functional enrichment analyses of the induced genes associated with lipid and surfactant production.
(C) Functional enrichment analyses of the suppressed genes show decreased gene expression associated with Wnt signaling, Immune and Mesenchyme development, Epithelial cell migration and proliferation and Epithelial cell differentiation.
(D) Immunofluorescence analysis of SOX9 (green) and SFTPC (white) demonstrates the presence of SOX9+/SFTPC+ epithelial cells in YAPdeleted mice. See Figure S3 for further ATAC-seq quality control.