Figure 2.

Comparison of cryosectioning with FIB milling

(A and B) (A) Tomographic slice through dividing S. pombe nucleus generated from a cryosectioned ribbon and (B) a cryo-FIB milled lamella, each ∼150-nm thick. In both slices, the nuclear membrane is visible near the center and is surrounding the microtubules (MT) of the mitotic spindle. Cytoplasmic vesicles (∗) and nuclear pore complexes (arrows) are also visible in both. While the general morphology is captured in both tomograms, the cryosection shows damage from compression (oval vesicles) and cracked membranes and MTs (arrowheads). Red arrows point to crevassing artifacts in the tomograms. Scale bars represent 100 nm.