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. 2021 Aug 8;24(9):102959. doi: 10.1016/j.isci.2021.102959

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Dynamic CLEM

(A) Top panel: Widefield image of GFP-labeled actomyosin rings during synchronized constriction. Dashed outlines show the rod-shaped body of S. pombe. Pairs of fluorescent puncta mark the optical cross section through the actomyosin ring (Swulius et al., 2018). Bottom panel: Side (left) and top (right) views of a 3D model of the membrane (blue) and actin filaments (orange). Arrowheads point to filament ends.

(B) 3D model of ARP2/3-initiated actin wave extracted from a cryotomogram. The color of the filament represents the divergence of its angle from a flat position (Jasnin et al., 2019). Scale bar in the bottom panel of (A) is 100 nm.