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Integrated approaches
(A) Live-cell fluorescence image of EM grid with cells growing on it (red) at the end of imaging session in MAVIS (Koning et al., 2014).
(B) Cryo-fluorescence image of the same cells after blotting and plunge-freezing.
(C) DIC image of C. elegans within the microfluidic chamber before and after freezing.
(D) Lamella from vitrified C. elegans during cryo lift out (Fuest et al., 2019). Scale bars in (C) and (D) are 30 μm and 20 μm, respectively.
