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. 2021 Aug 8;24(9):102959. doi: 10.1016/j.isci.2021.102959

Figure 9.

Figure 9

Hardware developments for cryo-ET

(A and B) Movie frames of viral particles were collected on a direct electron detector. Translational alignment of movie frames, as in B, preserves high-resolution information, unlike in the unaligned image in A (Brilot et al., 2012).

(C and D) Single-tilt images of a neurite imaged in a TEM near focus (500-nm under focus) with (D) or without (C) a Volta phase plate. Contrast is much higher with the use of the phase plate, as microtubules and membrane can be seen in (D), but not in (C). Scale bars in (C) and (D) are 100 nm.