Figure 2.

circOMA1 promotes the tumorigenesis of BC. (A) MCF-7 cells were transfected as indicated and the expression of circOMA1 was evaluated by RT-qPCR. (B) MDA-MB-468 cells were transfected with pcDNA3.1 or pcDNA.circOMA1, and the expression of circOMA1 was measured by RT-qPCR. (C) BC cells were transfected as indicated and the proliferation of cells was measured by Cell Counting Kit-8 assay at different time points. (D) BC cells were transfected as indicated and cellular migration was assessed (magnification, ×100). (E) BC cells were transfected as indicated and cellular invasion was assessed (magnification, ×100). (F) BC cells were transfected as indicated and cellular apoptosis was assayed. Data are presented as the mean ± SD. Experiments were performed at least three times. *P<0.05, **P<0.01. BC, breast cancer; circ, circular RNA; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin RNA; NC, negative control; OD, optical density; PI, propidium iodide.