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. 2021 Aug 10;12:721887. doi: 10.3389/fimmu.2021.721887

Figure 1.

Figure 1

ROS formation in human whole blood samples. ROS formation in neutrophils (A), classicals (B) and non-classicals (C) was assessed after incubation with 50 µg/mL pCRP and mCRP, 20 µM ADP, 10 µg/mL LPS, respectively, or stayed unstimulated (control). We observed a significant increase in ROS generation in neutrophils and both monocyte subsets when treated with 25 µg/mL mCRP, but not 50 µg/mL pCRP ***P < 0.001 and **** < 0.0001, (A–C). Cells incubated with 20 µM ADP failed to raise ROS production significantly over the control level. LPS served as a positive control and showed the most substantial increase in ROS generation in all three leukocyte subsets (****P < 0.0001). When all three subsets were compared for agent-specific ROS generation, we found classical monocytes to produce a higher respiratory burst than in the non-classical subset. This difference was found in both LPS and mCRP-stimulated cells (D, F). However, LPS induced the strongest respiratory burst in the neutrophil subset (ns not significant, *P < 0.05, **** and P < 0.0001, (D). For pCRP-stimulated cells no significant difference was found (E). Results are presented as means and S.E.M., P values were calculated with ANOVA and Tukey post hoc test, n = 6, ns, not significant; *** and ****P < 0.001 and < 0.0001.