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. 2021 Aug 10;12:721887. doi: 10.3389/fimmu.2021.721887

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Copyright © 2021 Zeller, Bogner, Kiefer, Braig, Winninger, Fricke, Karasu, Peter, Huber-Lang and Eisenhardt

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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pCRP facilitates phagocytosis of zymosan-FITC targets by opsonization, while mCRP does not. Phagocytosis by neutrophils (A, B), classical monocytes (C, D), and non-classical monocytes (E, F) was assessed by the described flow cytometry protocol. pCRP added to FITC-tagged zymosan targets were engulfed significantly faster and in higher amounts compared to untreated controls (A, C, E). mCRP added in the same concentration showed no significant effects on the phagocytosis in the assessed subtypes (B, D, F). All results are presented as means and S.E.M. and P values were calculated with ANOVA and Tukey post hoc test, n = 6, ^#not significant, * and ** P < 0.05 and < 0.01.