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. 2021 Jul 21;297(3):100992. doi: 10.1016/j.jbc.2021.100992

Figure 1.

Figure 1

Prx forms stable complexes with various ComR orthologs.A, pull-down assay of Prx with various ComR orthologs including Type II ComR proteins (ComR S. mutans, ComR MGAS5005) Type I (ComR S. thermophilus) and Type III (ComR S. suis). Prx (with a His-tag) was first added to Ni-NTA resin to be used as bait, followed by incubation with purified ComR (no His-tag). Controls with beads without Prx (no Prx Controls) and purified protein (ComR controls) are shown. B, size-exclusion chromatography (SEC) binding assay with the ComR structural relative Rgg3. The top panel shows the chromatogram and the bottom panel a Coomassie-stained SDS-PAGE gel of the corresponding fractions.