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. 2021 Jul 21;297(3):100992. doi: 10.1016/j.jbc.2021.100992

Figure 2.

Figure 2

Prx binds the ComR DBD with high affinity.A, size-exclusion chromatography binding assays of Prx with the TPR domain of ComR S. mutans and B, Prx with the DBD of ComR S. mutans. ComR minimal domains TPR or DBD incubated with Prx are shown in gray, the TPR or DBD alone as black dashed lines, and Prx alone as a dotted gray line. The void volume of the column is approximately 8 ml. C, isothermal titration calorimetry was performed with Prx and full-length ComR and in (D) with Prx and the ComR DBD. E, isothermal titration calorimetry controls of Prx, full-length ComR, and the ComR DBD with buffer. Prx forms a 1:1 stoichiometric complex with both full-length ComR and the ComR DBD with a binding constant of 390 nM and 50 nM respectively.