Figure 4.

Conserved acidic residues in Prx are required for interaction with ComR.A, size-exclusion chromatography binding assays of Prx variants with wild-type ComR. The chromatogram of ComR alone is indicated by a dotted black line and the chromatogram of ComR incubated with wild-type Prx as a dashed black line. Each Prx variant assay is plotted in color. Starred residues indicate contacts shown in Figure 3A that when mutated result in a loss of complex formation. B, representative Coomassie-stained SDS-PAGE gels of SEC binding controls and a Prx protein variant with ComR. C, circular dichroism spectra controls of the Prx variants compared with wild-type Prx. The Prx variant color key and labeling are matched with panel A. D, ITC experiment and control for PrxD32A and full-length ComR.