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. 2021 Jul 21;297(3):100992. doi: 10.1016/j.jbc.2021.100992

Figure 8.

Figure 8

Prx binding to ComR does not affect the interaction with XIP.A, example of observed fluorescence at the dansyl emission for increasing concentrations of ComR. Black is buffer background, blue is 0 μM ComR, red is 0.125 μM ComR, green is 0.75 μM ComR, and brown is 15 μM ComR. B, binding curve of fluorescently labeled Dansyl-XIP with wild-type ComR S. mutans. A constant concentration of dansyl-XIP (0.2 μM) with increasing ComR was used as defined in the Experimental procedures section. C, competition assays for dansyl-XIP bound to ComR with unlabeled XIP (gray) and Prx (black). Prx and XIP were added at the same molar ratios to the ComR:dansyl-XIP complex. Increasing XIP displaces dansyl-XIP and Prx has no effect. D, fluorescence anisotropy measurements of Prx added to ComR:Dansyl-XIP demonstrating that Prx can bind the ComR:XIP complex. Error bars are representative of three separate experiments.