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. Author manuscript; available in PMC: 2021 Aug 24.
Published in final edited form as: Biochem Pharmacol. 2005 Aug 1;70(3):381–393. doi: 10.1016/j.bcp.2005.05.002

Fig. 7.

Fig. 7.

Influence of MRS2481 on TNFα-induced IκBα degradation and JNK phosphorylation in repaired IB3-1/S9 cells. (a) IB3-1/S9 cells were pretreated with either 5 or 10 μM MRS2481, or the less potent analogue MRS2485 for 6 h. The cells were then treated with 10 ng/ml TNFα for 15 min. Reaction mixtures were then subjected to polyacrylamide gel electrophoresis, blotted onto nitrocellulose, and probed with anti-IκBα antibody. The left-most “0” is an untreated condition. The inner “0” is upon the addition of TNFα only. (b) IB3-1/S9 cells were pretreated with either 5 or 10 μM MRS2481, or the less potent analogue MRS2485 for 6 h. The cells were then treated with 10 ng/ml TNFα for 15 min. Reaction mixtures were then subjected to polyacrylamide gel electrophoresis, blotted onto nitrocellulose, and probed with anti-phospho-JNK or anti-JNK1 antibodies. The left-most “0” is an untreated condition. The inner “0” is upon the addition of TNFα only.