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. 2021 Aug 10;12:692837. doi: 10.3389/fmicb.2021.692837

TABLE 1.

PCR primers (forward and reserve) used for the amplification of Cryptosporidium, Enterocytozoon bieneusi, and Giardia duodenalis in this study.

Species (genetic marker) Primers (5′-3′) Length (∼bp) References
Cryptosporidium TTC TAG AGC TAA TAC ATG CG 1,325 Xiao et al., 1999
(SSU rDNA) CCC ATT TCC TTC GAA ACA GGA Xiao et al., 2001
GGA AGG GTT GTA TTT ATT AGA TAA AG 830 Jiang et al., 2005
CTC ATA AGG TGC TGA AGG AGT A
E. bieneusi MSP-1 (TGA ATG KGT CCC TGT) 590 Katzwinkel-Wladarsch et al., 1996
(ITS) MSP-2B (GTT CAT TCG CAC TAC T)
MSP-3 (GGA ATT CAC ACC GCC CGT CRY TAT) 508
MSP-4B (CCA AGC TTA TGC TTA AGT CCA GGG AG)
G. duodenalis AL3543 (AAA TTA TGC CTG CTC GTC G) 605 Sulaiman et al., 2003
(tpi) AL3546 (CAA ACC TTT TCC GCA AAC C)
AL3544 (CCC TTC ATC GGT GGT AAC TT) 532
AL3545 (GTG GCC ACC ACT CCC GTG CC)