Table 2.

Concentration of FAAs (μg amino acid per g dried F. vesiculosus extract) using different solvent types (deionised water, 0.1 M HCl and 1% citric acid) and extraction times (1 and 2 h)

FAAs	Extraction conditions
	Deionised water		0.1 M HCl		Citric acid (1%)
	1 h	2 h	1 h	2 h	1 h	2 h
Asp	nd	481.55 ± 7.19	315.96 ± 9.57	314.86 ± 8.0343	3.44 ± 3.06	nd
Glu	112.92 ± 3.81	1469.95 ± 3.86	1142.62 ± 12.21	1157.92 ± 32.08	277.22 ± 57.99	15.86 ± 3.12
Ser	nd	22.42 ± 0.64	23.04 ± 4.88	28.45 ± 2.10	13.93 ± 8.82	nd
His	nd	nd	nd	2.59 ± 1.93	nd	3.19 ± 1.60
Gly	4.94 ± 0.53	62.11 ± 0.34	65.23 ± 2.64	67.08 ± 0.88	36.51 ± 10.41	30.17 ± 0.57
Thr	nd	44.42 ± 1.21	36.30 ± 2.06	38.79 ± 2.68	1.21 ± 1.21	nd
Arg	nd	48.14 ± 0.11	80.85 ± 6.10	91.13 ± 6.01	36.35 ± 9.70	nd
Ala	21.22 ± 0.86	574.75 ± 1.48	532.78 ± 7.37	514.24 ± 6.14	135.93 ± 9.82	142.29 ± 0.97
Tyr	nd	nd	nd	nd	nd	nd
Val	nd	829.95 ± 0.44	1000.35 ± 29.37	1007.01 ± 3.78	5.29 ± 2.39	nd
Met	nd	nd	nd	nd	nd	nd
Trp	nd	nd	nd	nd	nd	nd
Phe	nd	nd	98.19 ± 16.69	63.54 ± 3.89	nd	nd
Ile	nd	nd	nd	nd	nd	nd
Leu	nd	nd	nd	nd	2.38 ± 1.84	nd
Lys	nd	10.55 ± 0.45	23.16 ± 2.08	21.31 ± 1.01	71.30 ± 11.84	62.81 ± 2.11
Total FAAs	139.09 ± 5 C***	3543.89 ± 7.08 a	3318.54 ± 74.66 A	3306.97 ± 50.05 b	583.62 ± 112.92 B*	254.34 ± 4.62 c

Results are expressed as average ± standard deviation of the mean (n = 3). The abbreviation nd in the table indicates non-detected. Different letters indicate statistical differences in the amount of total FAAs extracted using multiple solvents during 1 h (uppercase letters) or 2 h (lowercase letters). The statistical differences in the amount of total FAAs extracted by using different times of extraction and the same solvent are represented as: *P < 0.05, **P < 0.01 and ***P < 0.001