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Expression and biochemical characterization of Acs1. (A) Reaction scheme of Acs1. (B) Progress curves for detecting the activity of purified recombinant Acs1 for six fungal enzymes using the EnzChek pyrophosphate detection kit (Thermo-Fisher). (C, D) Representative Michaelis–Menten curves for measuring substrate Km’s for CnAcs1; values for all enzymes reported in Table 1.
