FIGURE 2.

Pharmacological restoration of 5mC/5hmC levels in human GBM cell line LN-229. Cells were treated with demethylating agent 5-AZA-dC, either alone or in combination with vitamin C (Vit C). The effects on 5mC/5hmC levels and on KMT5B expression were assessed by (A) bisulfite pyrosequencing at two intragenic CpG positions (Wilcoxon rank sum test; *p < 0.05; **p < 0.01) and (B) KMT5B mRNA relative expression by means of qRT-PCR. The data are the average ±SEM of three independent experiments (Welch t-test; *p < 0.05; **p < 0.01).