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Immunofluorescence analysis of mono- and dimethylation of H4K20. Representative images of mock and KMT5B-transfected clones immunostained with (A) anti-H4K20me1 or (B) anti-H4K20me2 antibodies (green). In panels (C,D) representative images of KMT5B-transfected clones immunostained with (C) anti-H4K20me1 or (D) anti-H4K20me2 antibodies (green) after treating the cells with KMT5B inhibitor A-196 for 48 h. Nuclei were counterstained with DAPI (blue). Bars: 10 μm.
